Layer 1 of the ISO/OSI protocol stack is the physical layer, which provides the means of transmitting and receiving raw data bits over a physical medium. It defines the electrical and physical specifications of the data connection, such as the voltage levels, signal timing, cable types, connectors, and pin assignments. It does not perform any data encryption, routing, end-to-end connectivity, framing, error checking, or user applications. These functions are performed by higher layers of the protocol stack, such as the data link layer, the network layer, the transport layer, and the application layer. References: ISO/IEC 7498-1:1994, Section 6.11; ISA/IEC 62443 Cybersecurity Fundamentals Specialist Study Guide, Section 3.1.12