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American Society of Microbiology ABMM ABMM Question # 58 Topic 1 Discussion

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American Society of Microbiology ABMM ABMM Question # 58 Topic 1 Discussion

ABMM Exam Topic 1 Question 58 Discussion:
Question #: 58
Topic #: 1

A clinical microbiology laboratory is performing quality control on Gram stain reagents. A control slide containing Escherichia coli and Staphylococcus aureus shows both organisms staining purple after the complete staining procedure. The MOST likely cause of this error is:
A.

Over-decolorization with alcohol-acetone.

B.

Insufficient application of crystal violet.

C.

Omission of the iodine mordant.

D.

Insufficient washing after crystal violet.

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Actual exam question for American Society of Microbiology ABMM exam by Zephyr867 at Aug 4, 2025, 5:37:18 AM

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