Hashing files during chain of custody handling ensures integrity, which means that the files have not been altered or tampered with during the collection, preservation, or analysis of digital evidence1. Hashing is a process of applying a mathematical function to a file to generate a unique value, called a hash or a digest, that represents the file’s content. By comparing the hash values of the original and the copied files, the integrity of the files can be verified. Availability, accountability, and non-repudiation are not ensured by hashing files during chain of custody handling, as they are related to different aspects of information security. References: 1: CISSP All-in-One Exam Guide, Eighth Edition, Chapter 10, page 633.