From the Scanning and Rating Components lesson, the Study Guide explicitly states:

" The second section of the Scan Profile, VM Association, allows you to define file extensions and VM image associations. This means that specific files are sandboxed by the associated VM image. To assign a file to a VM image, the following conditions must be true:

The file type must be configured to enter the job queue (first section of the scan profile).

The VM image clone value cannot be a non-zero number. "

This directly confirms:

Option B — The VM image clone value must be a non-zero number (clones must be allocated)

Option C — The file type must be configured to enter the job queue via the scan profile Pre-Filter section

Options A and D, while potentially relevant in practice, are not listed as the two required conditions in the Study Guide.