American Society of Microbiology ABMM ABMM Question # 66 Topic 2 Discussion
ABMM Exam Topic 2 Question 66 Discussion:
Question #: 66
Topic #: 2
A clinical microbiology laboratory is using isothermal nucleic acid amplification techniques for rapid detection of Mycobacterium tuberculosis in sputum. Compared to conventional PCR, isothermal methods offer the advantage of:
A.
Higher sensitivity and specificity for detecting low levels of target DNA.
B.
Faster reaction times and the ability to be performed without sophisticated thermocyclers.
C.
The ability to simultaneously amplify multiple targets in a single reaction.
D.
Easier integration with downstream analysis techniques such as Sanger sequencing.
Chosen Answer:
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