American Society of Microbiology ABMM ABMM Question # 60 Topic 2 Discussion
ABMM Exam Topic 2 Question 60 Discussion:
Question #: 60
Topic #: 2
A centrifuge rotor containing capped tubes of sputum concentrate for AFB culture breaks during operation inside a Class II Biological Safety Cabinet (BSC). What is the most appropriate immediate action for the technologist?
A.
Immediately turn off the BSC, open the sash fully, and spray disinfectant widely.
B.
Keep the BSC running, notify the supervisor, evacuate the immediate area, and post warning signs. Allow aerosols to settle (e.g., 30 min) before initiating decontamination with a tuberculocidal disinfectant.
C.
Carefully remove any intact tubes, wipe them with 70% alcohol, and place them in a new rotor to continue processing.
D.
Open the centrifuge within the BSC, discard broken glass in a regular sharps container, and clean the spill with 10% bleach.
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