Option A is incorrect because creating an Amazon EventBridge rule that invokes an AWS Lambda function to query the applications on a 5-minute interval is not a valid solution. EventBridge rules can only trigger Lambda functions based on events, not on time intervals. Moreover, querying the applications on a 5-minute interval might incur unnecessary costs and network overhead, and might not detect performance issues in real time.

Option B is correct because creating an Amazon CloudWatch Synthetics canary that runs a custom script to query the applications on a 5-minute interval is a valid solution. CloudWatch Synthetics canaries are configurable scripts that monitor endpoints and APIs by simulating customer behavior. Canaries can run as often as once per minute, and can measure the latency and availability of the applications. Canaries can also send notifications to an Amazon SNS topic when they detect errors or performance issues1.

Option C is incorrect because creating an Amazon CloudWatch alarm that uses the AWS/ApplicationELB namespace RequestCountPerTarget metric is not a valid solution. The RequestCountPerTarget metric measures the number of requests completed or connections made per target in a target group2. This metric does not reflect the application response time, which is the requirement. Moreover, configuring the CloudWatch alarm to send a notification when the number of connections becomes greater than the configured number of threads that the application supports is not a valid way to measure the application performance, as it depends on the application design and implementation.

Option D is incorrect because creating an Amazon CloudWatch alarm that uses the AWS/ApplicationELB namespace RequestCountPerTarget metric is not a valid solution, for the same reason as option C. The RequestCountPerTarget metric does not reflect the application response time, which is the requirement. Moreover, configuring the CloudWatch alarm to send a notification when the average response time becomes greater than the longest response time that the application supports is not a valid way to measure the application performance, as it does not account for variability or outliers in the response time distribution.