The action that an administrator should take to ensure application.exe cannot run is to remove the Permissions rule for C:\Program Files\IT\Tools*. This is because the Permissions rule has a higher priority than the Blocking and Isolation rule, and it allows any operation on any file in that path, including application.exe. By removing the Permissions rule, the Blocking and Isolation rule will apply and terminate application.exe based on its SUSPECT_MALWARE reputation. The other options are incorrect because they will not prevent application.exe from running. Option A is incorrect because changing the reputation to KNOWN MALWARE will not override the Permissions rule that allows any operation on the file. Option B is incorrect because the file will not be blocked based on reputation alone, as the Permissions rule will bypass the reputation check. Option D is incorrect because adding the hash to the company banned list will not override the Permissions rule that allows any operation on the file. References: Precedence of Policy Rules, Set Permission Policy Rules, Set Blocking and Isolation Policy Rules