According to the AWS Cloud Operations and EC2 Connectivity documentation, EC2 Instance Connect Endpoint allows access to instances without internet exposure or open SSH ports. However, for successful connectivity, the EC2 instance must have Systems Manager permissions through an IAM instance profile.

If no IAM instance profile is attached, the instance cannot establish a control channel with the Systems Manager service, and EC2 Instance Connect cannot authenticate the session.

Opening port 22 (Option B) is unnecessary and contradicts the private subnet design. HTTPS rules (Option A) are irrelevant because EC2 Instance Connect communicates through AWS APIs, not direct HTTPS connections. Recreating the instance with a key pair (Option D) bypasses the intended keyless connection mechanism.

Therefore, Option C — attaching an IAM instance profile with Systems Manager permissions — enables secure, private access through EC2 Instance Connect Endpoint.