These are the possible causes for the AccessDenied error because they affect the permissions to access the S3 object from the EC2 instance. An S3 bucket policy is a resource-based policy that defines who can access the bucket and its objects, and what actions they can perform. An IAM role is an identity that can be assumed by an EC2 instance to grant it permissions to access AWS services and resources. If there is an error in the S3 bucket policy or the IAM role configuration, such as a missing or incorrect statement, condition, or principal, then the EC2 instance may not have the necessary permissions to download the object from the S3 bucket .

https://docs.aws.amazon.com/AmazonS3/latest/userguide/example-bucket-policies.html

https://docs.aws.amazon.com/AWSEC2/latest/UserGuide/iam-roles-for-amazon-ec2.html